Double staining in immunohistochemistry for pathology laboratories can be easily achieved using the "Zytomed Systems Double Stain Polymer Detection Kit (POLDS-006)" which uses a one-step detection technique.
The sequential protocol described above is a 2-step technique, and can therefore be longer, but just as easy.
Also, to carry out this double labeling, it is imperative to use in diagnosis a primary antibody of mouse and primary antibody of Rabbit.